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Abstract
Potato (Solanum tuberosum L.) is a tuberous crop from the Solanaceae family which is a source of starch and food to many in Nigeria, however, its production is being hampered in field and storage by bacterial pathogen which causes rot of the stored tubers. To this end, the study aimed at screening potatoes from different stores within Ibadan for the evaluation and diversity of bacteria pathogens responsible for rot in potatoes. Damaged stored potato samples were collected from different locations in Ibadan for studies, these samples were isolated for bacterial pathogen and characterized biochemically and observed under the microscope for identification. Three genera of bacteria were observed to be responsible for potato rot in the study and they include Pectobacterium carotovorum, Pseudomonas syringae and Ralstonia solanacearum. Of all the isolated bacteria, Pectobacterium carotovorum has the highest occurrence with a frequency of 60%, while Pseudomonas syringae has a frequency of 33% and Ralstonia solanacearum has a frequency of 27%. The pathogenicity of the isolates were tested and this revealed that Pectobacterium carotovorum is the most virulent with a severity score of 4.3, while Ralstonia solanacearum follows with a score of 3.3 and a score of 2.7 was recorded for Pseudomonas syringae, while the control had a score of 0. The diversity and differences shown in the isolated bacteria indicated that potato rot is a serious disease which is caused by different bacteria and need an integrated approach for its control from the field of harvesting to the storage house.
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References
Clark, Hoy, 1994. Identification of resistance in sweetpotato to Rhizopus soft rot using two inoculation methods. Plant disease (USA). https://doi.org/10.1094/pd-78-1078
Duche, Omoigui, Iheukwumere, 2015. Variations among Xanthomonas axonopodis pv. vignicola isolates in Benue State, Nigeria. International Journal of Innovation and Scientific Research 13, 271-8.
Echerenwa, Umechuruba, 2004. Post-harvest fungal diseases of pawpaw (Carica papaya L.) fruits and seeds in Nigeria. Global Journal of Pure and Applied Sciences 10, 69-73. https://doi.org/10.4314/gjpas.v10i1.16360
El-Habbak, Refaat, 2019. Molecular detection of the causative agent of the potato soft rot, Pectobacterium carotovorum, in Egypt and essential oils as a potential safe tool for its management. Egyptian Journal of Biological Pest Control 29, 5. https://doi.org/10.1186/s41938-019-0104-1
Faostat, 2018. Statistical data. Food and Agriculture Organization of the United Nations, Rome. In.
Frampton, Pitman, Fineran, 2012. Advances in bacteriophage-mediated control of plant pathogens. International journal of microbiology 2012. https://doi.org/10.1155/2012/326452
Gnanamanickam, 2006. Plant-associated bacteria. Springer. https://doi.org/10.1007/1-4020-4538-7
Kakuhenzire, Lemaga, Tibanyendera, et al. Positive selection: a simple technique for improving seed potato quality and potato productivity among smallholder farmers. Proceedings of the II All Africa Horticulture Congress 1007, 2012, 225-33. https://doi.org/10.17660/actahortic.2013.1007.22
Khatri-Chhetri, Wydra, Rudolph, 2003. Metabolic diversity of Xanthomonas axonopodis pv. vignicola, causal agent of cowpea bacterial blight and pustule. European Journal of Plant Pathology 109, 851-60. https://doi.org/10.15740/has/ijpp/7.2/292-297
Martin, French, 1985. Bacterial wilt of potato. International Potato Center.
Mcneill, 1999. How the potato changed the world's history. Social Research 66, 67.
Onuegbu, 2002. Fundamentals of Crop Protection, Agro services and extension unit. Rivers State University of Science and Technology, Nkpolu PortHarcourt, 190-1.
Pérombelon, 2002. Potato diseases caused by soft rot erwinias: an overview of pathogenesis. Plant pathology 51, 1-12. https://doi.org/10.1046/j.0032-0862.2001.short
Sas, 2002. SAS® software Version 9.3, User's Manual. In.: SAS Institute Cary, North Carolina.
Tanni, Jahan, Roy, Modak, Begum, 2019. Characterization of Bacterial Isolates Associated with Postharvest Decay of Potato in Bangladesh. Journal of Advances in Microbiology, 1-9. https://doi.org/10.9734/jamb/2019/45835
Tewe, Ojeniyi, Abu, 2003. Sweet potato production, utilisation and marketing in Nigeria. In.: The International Potato Center (CIP), Apartado 1558, Lima 12, Peru.
Toth, Bell, Holeva, Birch, 2003. Soft rot erwiniae: from genes to genomes. Molecular plant pathology 4, 17-30. https://doi.org/10.1046/j.1364-3703.2003.00149.x
Van Harsselaar, Lorenz, Senning, Sonnewald, Sonnewald, 2017. Genome-wide analysis of starch metabolism genes in potato (Solanum tuberosum L.). BMC genomics 18, 37. https://doi.org/10.1186/s12864-016-3381-z
Vauterin, Swings, Kersters, 1991. Grouping of Xanthomonas campestris pathovars by SDS-PAGE of proteins. Microbiology 137, 1677-87. https://doi.org/10.1099/00221287-137-7-1677
Verma, Meena, Kumar, 2018. Chemical and biological properties of the soil influenced by crop geometry and nutrient management practices in winter maize (Zea mays L.)+ potato (Solanum tuberosum L.) intercropping system. Journal of Pharmacognosy and Phytochemistry 7, 1952-6.
Vreugdenhil, Bradshaw, Gebhardt, et al., 2011. Potato biology and biotechnology: advances and perspectives. Elsevier. https://doi.org/10.1016/b978-044451018-1/50040-3
Wakil, Oyinlola, 2011. Diversity of pectinolytic bacteria causing soft rot disease of vegetables in Ibadan, Nigeria. Journal of Applied Biosciences 38, 2540-50.
Yenjerappa, 2009. Epidemiology and management of bacterial blight of pomegranate caused by Xanthomonas axonopodis pv. punicae (Hingorani and Singh) Vauterin et al: University of Horticulture and Forestry, India.Ph.D. Thesis.