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Profenophos is a commonly used organophosphate pesticide in pulse crops; however, it is difficult to say whether it is safe from cytogenotoxic effects. Test plant materials (field pea seeds) were soaked in 250 ml of 0.2%, 0.4% and 0.6% profenophos 50% EC separately for 6 hours. The mechanism behind various types of chromosomal anomalies observed due to treatment with profenophos has been discussed in detail.  The effects of the pesticide that appeared in M1 generation diluted in M2. The appearance of C-metaphase with univalent and bivalents, multipolarities, chromatin bridges suggest that these organophosphate pesticides like profenophos affect genetic recombination which may lead to loss of important factors, gain of undesirable characters. This study aims at finding the cytological and genotoxic effect of pesticide profenophos on germ cells of Pisum sativum var. arvense and suggests judicious means of application of pesticides and agrochemicals in appropriate condition to elude further damages in the future.

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Author Biography

Rupak Kumar Sengupta, City College, University of Calcutta, Kolkata 700009,West Bengal, India



How to Cite
Sengupta, A., & Sengupta, R. K. (2021). Genotoxic effects of profenophos on Pisum sativum. Journal of Agriculture and Applied Biology, 3(1), 1-7.


Aksoy, Ö., Asuman, D., & Tütünoğlu, B. (2015). Genotoxic effects of the fungicide Sportak on Zea mays. Fresenius Environmental Bulletin, 24(2): 552–559. Direct Link.
Bonciu, E. (2012). Cytological effects induced by Agil herbicide to onion. Journal of Horticulture, Forestry and Biotechnology, 16:68-72. Direct Link.
Choudhary, S., & Sajid, S. (1986). The behaviour of mei-otic chromosomes as revealed through the use of Bavistin on pea. Cytologia, 51(2), 279-288. Cross-Ref
Darlington, C.D. (1953) Symposium on chromosome breakage. Heridity,6,1-315. Direct Link.
Food and Agriculture Organization. (2019). FAOSTAT 2019. Direct Link.
Fuchs, L., Jenkins, G., & Phillips, D. (2018). Anthropogen-ic impacts on meiosis in plants. Frontiers in Plant Science, 9. CrossRef
Haiba, A. A. A., El-hamid, N. R. A., El-hady, E. A. A. A., & Al-ansary, E. R. M. F. (2012). Cytogenetic effect of in-secticide telliton and fungicide dithane M-45 on meiotic cells and seed storage proteins of Vicia fa-ba. Journal American Science, 7(1):19–25. Direct Link.
Jain, A., & Sarbhoy, R. (1988). Cytogenetical studies on the effects of some chlorinated pesticides. III. con-cluding remarks. Cytologia, 53(3), 427-436. Cross-Ref
Kuchy, A., Wani, A., & Kamili, A. (2015). Cytogenetic ef-fects of three commercially formulated pesticides on somatic and germ cells of Allium ce-pa. Environmental Science and Pollution Re-search, 23(7), 6895-6906. CrossRef
Kumar, P., Leela, K., Laxminarayana, P., & Nizam, J. (1978). Induction of multipolar spindle in Allium sativum. Cytobios, 22(85), 41–45.
Maity, S. (2014). Effects of Dithane M-45 (A fungicide) on root meristem of Vigna mungo (L.) hep-per. International Journal of Advanced Research in Engineering and Applied Sciences, 3:1-6. Direct Link.
Mason, A., & Pires, J. (2015). Unreduced gametes: meiot-ic mishap or evolutionary mechanism?. Trends In Genetics, 31(1), 5-10. CrossRef
Ouyang, Y., Tang, H., Wu, Y., & Li, G. (2013). Rapid de-termination of pesticide multiresidues in vegeta-bles and fruits by accelerated solvent extraction coupled with online gel permeation chromatog-raphy-gas chromatography-mass spectrome-try. Chinese Journal Of Chromatography, 30(7), 654-659. CrossRef
Pulate, P. V., & Tarar, J. L. (2014). Cytogenetic effect of systemic fungicide calixin on root meristem cells of Allium cepa L. International Journal of Life Sci-ences, 2(4):341- 345. Direct Link
Selvaraju, S., Vasanth, M., Rajarajan, R., Muralidharan, R., & Raghupathy, V. (2015). Genotoxic effects of car-bendezim (fungicide) on the root apical meristems of Allium cepa L. Research Journal of Pharmacog-nosy and Phytochemistry, 7(1), 29. CrossRef
Shahzad, B., Tanveer, M., Che, Z., Rehman, A., Cheema, S. A., Sharma, A., Song, H., Rehman, S. U., & Zhaorong, D. (2018). Role of 24-epibrassinolide (EBL) in me-diating heavy metal and pesticide induced oxida-tive stress in plants: A review. Ecotoxicology And Environmental Safety, 147, 935-944. CrossRef
Sharma, A., Thakur, S., Kumar, V., Kanwar, M., Kesavan, A., Thukral, A., Bhardwaj, R., Alam, P., & Ahmad, P. (2016). Pre-sowing seed treatment with 24-epibrassinolide ameliorates pesticide stress in Brassica juncea L. through the modulation of stress markers. Frontiers In Plant Science, 7. CrossRef