The efficacy of Acorus calamus L. crude extract formulated in bentonite nanoparticles against Crocidolomia pavonana (Lepidoptera:Pyralidae)
DOI:
https://doi.org/10.11594/jaab.04.02.07Keywords:
Antifeedant, β-asarone, Na-bentonite, Phytotoxic effect, Vulnerability to UVAbstract
The prospect of A. calamus extract to be botanical insecticides to control the insect pests is very promising. The insect control properties of the A. calamus are predominantly to have β-asarone, saponin and flavonoid. However, there are some factors limiting their efficacy. Short release rate at the point contact, the inherent volatility and vulnerability to oxidation and ultra-violet light are causing phytochemical changes during the application. Thus developing nanotechnology to increase their efficacy studied in this research. To develop the plant extract in nanoformulation, experiment on adsorption and desoption capacity were carried out with Acorus extract treated bentonite substrate analysed by GC techniques. In addition, to investigate the effectiveness of the plant extract treated bentonite were evaluated on Crocidolomia pavonana. Third instar larvae were used in the evaluation of antifeedant effect by using no choice methods. The results showed that Bentonite nanoparticles was effective in controlling the release of a bioactive element. Purifed clay bentonite for getting Na-bentonite increased the specific surface area of material therefore increased the adsorption site on the clay layer of the material. Crude extract of A.calamus can dissolve well in combination of water and organic solvents. Desorption experiments proved that Na-bentonite released more slowly than raw clay bentonite. Purification of bentonite to Na-bentonite resulted the changing of phytochemical properties bentonite which lead to an increase in the adsorption capacity of bentonite. In term of antifeedant evaluation based on damage to cabbage leaves seedling, the least damage was observed on leaves with nanoparticle plant extract formulation. The antifeedant index of A. calamus in nanoparticles formulation showed 25% higher that untreated cabbage plant seedling. The glasshouse trial was conducted to evaluate their efficacy on cabbage seedling. However, the brown leaves or phytotoxic effect has been found by the time the extracts nano formulation has been sprayed.
Downloads
References
Hossain, M. S., Zaman, S., Haque, A. B. M. H., Bhuiyan, M. P. I., Khondkar, P., & Islam, M. R. (2008). Chemical and pesticidal studies on Acorus calamus rhizomes. Journal of Applied Sciences Research, 4(10), 1261–1266. Direct Link.
Jiang, H., Chen, Y., Ni, J., Song, J., Li, L., Yu, Z., Pang, L., & Qi, H. (2020). Biphasic dose–response of components from coptis chinensis on feeding and detoxification enzymes of Spodoptera litura larvae. Dose-Response, 18(3), 1–8. CrossRef
Koul, O. (1987). Antifeedant and growth inhibitory effects of calamus oil and neem oil on Spodoptera litura under laboratory conditions. Phytoparasitica, 15(3), 169–180. CrossRef
Kumarawati, N. P. N., Supartha, I. W., & Yuliadhi, K. A. (2013). Struktur komunitas dan serangan hama-hama penting tanaman kubis (Brassica oleracea L.). E-Jurnal Agroekoteknologi Tropika (The E-Journal of Tropical Agroecotechnology), 2(4), 252–259. Direct Link.
Kumrungsee, et al. (2023). Phenolic secondary metabolites from Acorus calamus (Acorales: Acoraceae) rhizomes: the feeding deterrents for Spodoptera litura (Lepidoptera: Noctuidae). Journal of Economic Entomology, 116(5), 1613–1620. CrossRef
Liu, X. C., Zhou, L. G., Liu, Z. L., & Du, S. S. (2013). Identification of insecticidal constituents of the essential oil of Acorus calamus rhizomes against Liposcelis bostrychophila badonnel. Molecules, 18(5), 5684–5696. CrossRef
Masini, J. C., & Abate, G. (2021). Guidelines to study the adsorption of pesticides onto clay minerals aiming at a straightforward evaluation of their removal performance. Minerals, 11(11). CrossRef
Murugan, K., Roni, M., Panneerselvam, C., Aziz, A. T., Suresh, U., Rajaganesh, R., Aruliah, R., Mahyoub, J. A., Trivedi, S., Rehman, H., Naji Al-Aoh, H. A., Kumar, S., Higuchi, A., Vaseeharan, B., Wei, H., Senthil-Nathan, S., Canale, A., & Benelli, G. (2018). Sargassum wightii-synthesized ZnO nanoparticles reduce the fitness and reproduction of the malaria vector Anopheles stephensi and cotton bollworm Helicoverpa armigera. Physiological and Molecular Plant Pathology, 101, 202–213. CrossRef
Pavela, R., Maggi, F., Iannarelli, R., & Benelli, G. (2019). Plant extracts for developing mosquito larvicides: From laboratory to the field, with insights on the modes of action. Acta Tropica, 193, 236–271. CrossRef
Pavoni, L., Pavela, R., Cespi, M., Bonacucina, G., Maggi, F., Zeni, V., Canale, A., Lucchi, A., Bruschi, F., & Benelli, G. (2019). Green micro-and nanoemulsions for managing parasites, vectors and pests. Nanomaterials, 9(9). CrossRef
Purwatiningsih, B., Rumhayati, B., Fajariyah, S., & Jannah, R. (2023). The effectiveness of suspension beta-asanore mixed with silica nanoparticles in the mortality of Crocodolomia pavonana. In Sugiharto, B., Lelono, A., Bahar, M.A., Wathon, S., Senjarini, K., Arip, A.G., Putrasetya, R., Anika, B., Sukma, A.A. (Eds.), Proceedings of the 4th International Conference on Life Sciences and Biotechnology (ICOLIB 2021) (Vol. 1). Atlantis Press International BV. CrossRef
Purwatiningsih, P. (2013). Evaluation of Insecticidal Efficacy of Acorus calamus L. and Leptospermum petersonii FM. Bailey on Plutella xylostella L. (Unpublished doctoral thesis, University of Queensland), Australia
Purwatiningsih, P., Heather, N., & Errol, H. (2012). Efficacy of Leptospermum petersonii oil, on Plutella xylostella, and its parasitoid, Trichogramma pretiosum. Journal Economic Entomology ., 105(4), 1379–1384. CrossRef
Schmutterer, H. (1990). Properties and potential of natural pesticides from the neem tree, Azadirachta indica. Annual Review of Entomology, 35(1), 271–297. CrossRef
Sembiring, J., & Prasetia, A. (2019). Pest attack on cabbage and mustard greens in Tanah miring district, Merauke Regency, Papua Province. International Journal of Civil Engineering and Technology, 10(2), 773–782. Direct Link.
Teshale Degefu. (2012). A study of the efficacy of Australian phytochemicals in clay nanoparticles as pesticides against Helicoverpa armigera (Hubner) and Myzus persicae (Sulzer). (Unpublished doctoral thesis, University of Queensland), Australia
Yuliadhi, K. A., Supartha, I. W., Wijaya, I. N., Pudjianto, Nurmansyah, A., Susila, I. W., Yudha, I. K. W., Utama, I. W. E. K., & Wiradana, P. A. (2021). Short communication: The preference and functional response of Sycanus aurantiacus (Hemiptera: Heteroptera: Reduviidae) on three prey types in laboratory conditions. Biodiversitas, 22(12), 5662–5667. CrossRef
Downloads
Published
Issue
Section
License
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See the Effect of Open Access).
