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Apr 15, 2024
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Jun 19, 2024
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Abstract
The dearth of information surrounding the utilization of Opuntia cladode alkaloids underscores a critical gap in understanding their pharmacological properties and therapeutic potential, emphasizing the need for further comprehensive investigations. The present study aims to investigate the in vitro anti-inflammatory, anticoagulant, and antimicrobial activities of alkaloïds belonging to young cladodes (nopals) of inermis Algerian Opuntia ficus indica. The assessed alkaloïds showed a moderate anti-inflammatory effect regarding the BSA protein protection with a maximum of 51.04±1.84 % compared to 84.22±2.38 % for Diclofenac sodium as a positive standard. However, their stabilization of red blood cells membrane against induced hemolysis was greater than Diclofenac (52.38±2.01 % vs 48.97±2.73 %). The two assays had a significant correlation (< 0.05) of 0,968. Nopal alkaloïds extended the coagulation time (1.24 fold) by significantly affecting the exogenous pathway PT only, whilst they were ineffective against the endogenous pathway APTT. Conversely, Algerian nopal alkaloïds had neither bacteriostatic nor bactericide influence at 2 mg ml-1 on Candida albicans yeast or the six tested pathogenic bacterial strains. In perspective, the purification of these Opuntia nopal alkaloïds and understanding their accurate mechanism of action are clearly the strategic steps to illustrate their overall curative potential.
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This work is licensed under a Creative Commons Attribution 4.0 International License.
How to Cite
Moussaoui, B., Hanafi, T., Rahali, A., Guemou, L., Reghioui, B., Zemour, K., & Riazi, A. (2024). In vitro anti-inflammatory and anticoagulant activities of alkaloïds extracted from nopals of inermis Algerian Opuntia ficus indica (L). Journal of Agriculture and Applied Biology, 5(1), 86-96. https://doi.org/10.11594/jaab.05.01.07
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Balouiri, M., Sadiki, M., & Ibnsouda, S. K. (2016). Methods for in vitro evaluating antimicrobial activity: A review. Journal of Pharmaceutical Analysis, 6(2), 71-79. CrossRef
Barbieri, R., Coppo, E., Marchese, A., Daglia, M., Sobarzo-Sánchez, E., Nabavi, S. F., & Nabavi, S. M. (2017). Phytochemicals for human disease: An update on plant-derived compounds an-tibacterial activity. Microbiological Research, 196, 44-68. CrossRef
Belhadj Slimen, I., Najar, T., & Abderrabba, M. (2016). Opuntia ficus-indica as a Source of Bioac-tive and Nutritional Phytochemicals. Journal of Food and Nutrition Sciences. 4(6), 162-169. CrossRef
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Chaouch, M. A., Hammi, K. M., Dhahri, M., Ben Mansour, M., Maaroufi, M. R., Le Cerf, D., & Maj-doub, H. (2018). Access to new anticoagulant by sulfation of pectin-like polysaccharides isolated from Opuntia ficus indica cladodes. International Journal of Biological Macromole-cules, 120, 1794–1800. CrossRef
Dos Santos, R., Pimenta-Freire, G., & Dias-Souza, M. V. (2015). Carotenoids and flavonoids can impair the effectiveness of some antimicrobial drugs against clinical isolates of Escherichia coli and Staphylococcus aureus. International Food Research Journal, 22(5), 1777-1782. CrossRef
Drăgan, M., Stan, C.D., Pânzariu, A., & Profire, L. (2016). Evaluation of anti-inflammatory poten-tial of some new ferullic acid derivatives. Farmacia, 64(2), 194-197. CrossRef
Ghumre, S.V., Sawant, M.G., Jadhav, V.M., Kadam, V.J., Sonawane, N., & Ramaiya, M. (2017). As-sessment of in-vitro anti-inflammatory activity of cynodon dactylon and acyclovir showing synergistic effect by albumin denaturation and membrane stabilization assay. Modern Ap-proaches in Drug Designing. 1(2), 18-24. CrossRef
Heuzé, V., and Tran, G. (2017). Prickly pear (Opuntia ficus-indica). Feedipedia, a programme by INRA, CIRAD, AFZ and FAO. CrossRef
Jayakumar, K., Meenu Krishnan, V.G., & Murugan, K. (2016). Evaluation of antioxidant and anti-hemolytic activities of purified caulophyllumine-A from Solanum mauritianum Scop. Jour-nal of Pharmacognosy and Phytochemistry, 5(2), 195-199. Direct Link.
Khouya, T., Ramchoun, M., Hmidani, A., Amrani, S., Harnafi, H., Benlyas, M., Filali Zegzouti, Y., & Alem, C. (2015). Anti-inflammatory, anticoagulant and antioxidant effects of aqueous ex-tracts from Moroccan thyme varieties. Asian Pacific Journal of Tropical Biomedicine; 5(8), 636–644. CrossRef
Lazzaroni, M. G., Piantoni, S., Masneri, S., Garrafa, E., Martini, G., Tincani, A., Andreoli, L., & Fran-ceschini, F. (2021). Coagulation dysfunction in COVID-19: The interplay between inflamma-tion, viral infection and the coagulation system. Blood Reviews, 46, 100745. CrossRef
Moussaoui, B., Rahali, A., Hamed, D. Guemou, L., & Riazi, A. (2022). Antioxidant and cytotoxic activities of alkaloids extracted from inermis nopals of Algerian Opuntia ficus-indica (L). Asian Journal of Agriculture and Biology. 2022(2): 202105231. CrossRef
Oyedapo, O. O., Akinpelu, B. A., Akinwunmi, K. F., Adeyinka, M. O., & Sipeolu, F. O. (2010). Red blood cell membrane stabilizing potentials of extracts of Lantana camara and its fractions. International Journal of Plant Physiology and Biochemistry, 2(4), 46-51. Direct Link.
Oyedapo, O.O., Famurewa, A.J. (1995). Antiprotease and membrane stabilizing activities of ex-tracts of Fagara zantho~Lvloides, Olax subscorpioides and Tetrapleura tetraptera. Interna-tional Journal of Pharmacognosy, 33, 65-69. CrossRef
Özçelik, B., Kartal, M., & Orhan, I. (2011). Cytotoxicity, antiviral and antimicrobial activities of alkaloids, flavonoids, and phenolic acids. Pharmaceutical Biology, 49(4), 396-402. CrossRef
Ramli, A. N. M., Manap, N. W. A., Bhuyar, P., & Azelee, N. I. W. (2020). Passion fruit (Passiflora edulis) peel powder extract and its application towards antibacterial and antioxidant activi-ty on the preserved meat products. SN Applied Sciences, 2, 1-11. CrossRef
Sánchez, E., Dávila‐Aviña, J., Castillo, S. L., Heredia, N., Vázquez‐Alvarado, R., & García, S. (2014). Antibacterial and antioxidant activities in extracts of fully grown cladodes of 8 cultivars of cactus pear. Journal of Food Science, 79(4), M659-M664. CrossRef
Stintzing, F. C., and Carle, R. (2005). Cactus stems (Opuntia spp.): A review on their chemistry, technology, and uses. Molecular Nutrition and Food Research, 49(2), 175–194. CrossRef
Suryawanshi, P., and Vidyasagar, G.M. (2016). Phytochemical screening for secondary metabo-lites of Opuntia dillenii Haw. Journal of Medicinal Plants Studies, 4(5), 39-43. Direct Link.
Tatti, P.N., Anitha, S., Shashidhara, S., Deepak, M., & Bidari, S. (2012). Evaluation of in-vitro anti-denaturation activity of isolated compound of butea monosperma bark. Pharma science monitor, 3(4). Direct Link.
Vinchurkar, A., Valsange, A., Dama, L., Sonawane, S., Gaikwad, N., Mane, P., & Dama, S. B. (2014). Evaluation of in-vitro anti-inflammatory activity of crude Lawsonia inermis leaf extract us-ing egg albumin denaturation assay. Trends Biotechnol Res, 8(33), 44. Direct Link.
Yubin, J.I., Miao, Y., Bing, W., & Yao, Z. (2014). The extraction, separation and purification of al-kaloïds in the natural medicine. Journal of Chemical and Pharmaceutical Research, 6(1), 338-345. Direct Link.
Alamgeer, Hasan, U.H., Uttra, A.M., & Rasool, S. (2015). Evaluation of in vitro and in vivo anti-arthritic potential of Berberis calliobotrys. Bangladesh Journal of Pharmacology, 10, 807–819. CrossRef
Al-Sehemi, A. G., Irfan, A. (2017). Effect of donor and acceptor groups on radical scavenging ac-tivity of phenol by density functional theory. Arabian Journal of Chemistry. 10(2), 1703-1710. CrossRef
Aruwa, C. E., Amoo, S. O., & Kudanga, T. (2018). Opuntia (Cactaceae) plant compounds, biological activities and prospects–A comprehensive review. Food Research International, 112, 328-344. CrossRef
Aruwa, C. E., Amoo, S. O., & Kudanga, T. (2019). Extractable and macromolecular antioxidants of Opuntia ficus-indica cladodes: Phytochemical profiling, antioxidant and antibacterial activi-ties. South African Journal of Botany, 125, 402-410. CrossRef
Athukorala, Y., Lee, K.W., Kim, S.K., & Jeon, Y.J. (2007). Anticoagulant activity of marine green and brown algae collected from Jeju Island in Korea. Bioresource Technology, 98, 1711–1716. CrossRef
Balouiri, M., Sadiki, M., & Ibnsouda, S. K. (2016). Methods for in vitro evaluating antimicrobial activity: A review. Journal of Pharmaceutical Analysis, 6(2), 71-79. CrossRef
Barbieri, R., Coppo, E., Marchese, A., Daglia, M., Sobarzo-Sánchez, E., Nabavi, S. F., & Nabavi, S. M. (2017). Phytochemicals for human disease: An update on plant-derived compounds an-tibacterial activity. Microbiological Research, 196, 44-68. CrossRef
Belhadj Slimen, I., Najar, T., & Abderrabba, M. (2016). Opuntia ficus-indica as a Source of Bioac-tive and Nutritional Phytochemicals. Journal of Food and Nutrition Sciences. 4(6), 162-169. CrossRef
Bhuyar, P., Rahim, M. H., Sundararaju, S., Maniam, G. P., & Govindan, N. (2020). Antioxidant and antibacterial activity of red seaweed Kappaphycus alvarezii against pathogenic bacte-ria. Global Journal of Environmental Science and Management, 6(1), 47-58. CrossRef
Chaouch, M. A., Hammi, K. M., Dhahri, M., Ben Mansour, M., Maaroufi, M. R., Le Cerf, D., & Maj-doub, H. (2018). Access to new anticoagulant by sulfation of pectin-like polysaccharides isolated from Opuntia ficus indica cladodes. International Journal of Biological Macromole-cules, 120, 1794–1800. CrossRef
Dos Santos, R., Pimenta-Freire, G., & Dias-Souza, M. V. (2015). Carotenoids and flavonoids can impair the effectiveness of some antimicrobial drugs against clinical isolates of Escherichia coli and Staphylococcus aureus. International Food Research Journal, 22(5), 1777-1782. CrossRef
Drăgan, M., Stan, C.D., Pânzariu, A., & Profire, L. (2016). Evaluation of anti-inflammatory poten-tial of some new ferullic acid derivatives. Farmacia, 64(2), 194-197. CrossRef
Ghumre, S.V., Sawant, M.G., Jadhav, V.M., Kadam, V.J., Sonawane, N., & Ramaiya, M. (2017). As-sessment of in-vitro anti-inflammatory activity of cynodon dactylon and acyclovir showing synergistic effect by albumin denaturation and membrane stabilization assay. Modern Ap-proaches in Drug Designing. 1(2), 18-24. CrossRef
Heuzé, V., and Tran, G. (2017). Prickly pear (Opuntia ficus-indica). Feedipedia, a programme by INRA, CIRAD, AFZ and FAO. CrossRef
Jayakumar, K., Meenu Krishnan, V.G., & Murugan, K. (2016). Evaluation of antioxidant and anti-hemolytic activities of purified caulophyllumine-A from Solanum mauritianum Scop. Jour-nal of Pharmacognosy and Phytochemistry, 5(2), 195-199. Direct Link.
Khouya, T., Ramchoun, M., Hmidani, A., Amrani, S., Harnafi, H., Benlyas, M., Filali Zegzouti, Y., & Alem, C. (2015). Anti-inflammatory, anticoagulant and antioxidant effects of aqueous ex-tracts from Moroccan thyme varieties. Asian Pacific Journal of Tropical Biomedicine; 5(8), 636–644. CrossRef
Lazzaroni, M. G., Piantoni, S., Masneri, S., Garrafa, E., Martini, G., Tincani, A., Andreoli, L., & Fran-ceschini, F. (2021). Coagulation dysfunction in COVID-19: The interplay between inflamma-tion, viral infection and the coagulation system. Blood Reviews, 46, 100745. CrossRef
Moussaoui, B., Rahali, A., Hamed, D. Guemou, L., & Riazi, A. (2022). Antioxidant and cytotoxic activities of alkaloids extracted from inermis nopals of Algerian Opuntia ficus-indica (L). Asian Journal of Agriculture and Biology. 2022(2): 202105231. CrossRef
Oyedapo, O. O., Akinpelu, B. A., Akinwunmi, K. F., Adeyinka, M. O., & Sipeolu, F. O. (2010). Red blood cell membrane stabilizing potentials of extracts of Lantana camara and its fractions. International Journal of Plant Physiology and Biochemistry, 2(4), 46-51. Direct Link.
Oyedapo, O.O., Famurewa, A.J. (1995). Antiprotease and membrane stabilizing activities of ex-tracts of Fagara zantho~Lvloides, Olax subscorpioides and Tetrapleura tetraptera. Interna-tional Journal of Pharmacognosy, 33, 65-69. CrossRef
Özçelik, B., Kartal, M., & Orhan, I. (2011). Cytotoxicity, antiviral and antimicrobial activities of alkaloids, flavonoids, and phenolic acids. Pharmaceutical Biology, 49(4), 396-402. CrossRef
Ramli, A. N. M., Manap, N. W. A., Bhuyar, P., & Azelee, N. I. W. (2020). Passion fruit (Passiflora edulis) peel powder extract and its application towards antibacterial and antioxidant activi-ty on the preserved meat products. SN Applied Sciences, 2, 1-11. CrossRef
Sánchez, E., Dávila‐Aviña, J., Castillo, S. L., Heredia, N., Vázquez‐Alvarado, R., & García, S. (2014). Antibacterial and antioxidant activities in extracts of fully grown cladodes of 8 cultivars of cactus pear. Journal of Food Science, 79(4), M659-M664. CrossRef
Stintzing, F. C., and Carle, R. (2005). Cactus stems (Opuntia spp.): A review on their chemistry, technology, and uses. Molecular Nutrition and Food Research, 49(2), 175–194. CrossRef
Suryawanshi, P., and Vidyasagar, G.M. (2016). Phytochemical screening for secondary metabo-lites of Opuntia dillenii Haw. Journal of Medicinal Plants Studies, 4(5), 39-43. Direct Link.
Tatti, P.N., Anitha, S., Shashidhara, S., Deepak, M., & Bidari, S. (2012). Evaluation of in-vitro anti-denaturation activity of isolated compound of butea monosperma bark. Pharma science monitor, 3(4). Direct Link.
Vinchurkar, A., Valsange, A., Dama, L., Sonawane, S., Gaikwad, N., Mane, P., & Dama, S. B. (2014). Evaluation of in-vitro anti-inflammatory activity of crude Lawsonia inermis leaf extract us-ing egg albumin denaturation assay. Trends Biotechnol Res, 8(33), 44. Direct Link.
Yubin, J.I., Miao, Y., Bing, W., & Yao, Z. (2014). The extraction, separation and purification of al-kaloïds in the natural medicine. Journal of Chemical and Pharmaceutical Research, 6(1), 338-345. Direct Link.